Inside of a machine with brightly colored lights
| Core Facilities

Flow Cytometry Core at HSCRB

Image credit: Joi Ito, Wikimedia Commons

Instruments and Experimental Set-up

The HSCRB-HSCI Flow Cytometry Core includes the following instruments for mammalian flow cytometry and cell sorting:

  • 15-parameter BD FACSAria II “Norman” for four-way sorting and analysis. For self-use and mid-range cell sorting.
  • 15-parameter BD FACSAria II+ “Mimi” and “Bertha” for four-way sorting and analysis. Customized for small particle to average cell (<0.1 microns) sorting.
  • 15- parameter BD LSR II for analysis.
  • Apple Mac Pros with Flo Jo for analysis.

The Core is dedicated to mammalian cell sorting and analysis. For non-mammalian FACS services, please see the FAS Systems Biology Flow Cytometry Core located in the Northwest Building on the Cambridge campus.

Services and Fees

The following usage fee schedules will take effect as of January 1, 2019, subject to change as Core usage and resources change. For Harvard internal Investigators with a valid Harvard Account Code and all HSCI members:

FACS-Aria (operator-assisted)/Training $114/hour
FACS-Aria (self-operated) $70/hour
FACS-LSR II (self-operated) $25/hour

For all other Investigators at Harvard Affiliates or other not-for-profit institutions:

FACS-Aria (operator-assisted)/Training $193/hour
FACS-Aria (self-operated) $118/hour
FACS-LSR II (self-operated) $42/hour

Rates for users from for-profit companies are $250/hour. Users who do not have a Harvard account code to directly charge are required to present a purchase order before services are provided.

Hours of Operation

The HSCRB Flow Cytometry Core facility is available 24/7 for self-service to those who have completed the requisite training and have scheduled time on the instruments.

Technician-assisted sorting and training are available Monday to Friday from 10 a.m. to 6 p.m.

To schedule technician-assisted or self-service time on the instruments, users must first request a username and password at our affiliated Joslin Diabetes Center website.

Please contact Joyce LaVecchio for information regarding training and procedures.

Operations and Staff

Joyce LaVecchio and Nema Kheradmand are available to perform sorting and analysis and assist researchers who are approved to self-sort.

Please let contact any of our operators to answer your questions about available services, training, scheduling equipment, work requests, billing, and technical issues regarding flow cytometry, FACS sorting, and analysis.

A FACS Faculty Steering Committee composed of Drs. Amy Wagers, Douglas Melton, and Jeffrey Macklis advise the operation of the departmental facility.

Contact

Please send any questions or feedback to Joyce LaVecchio.

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