Magavi SS, Macklis JD. Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo. Methods Mol Biol. 2008; 438:335-43.

Citation

Magavi SS, Macklis JD. 2008. Identification of newborn cells by BrdU labeling and immunocytochemistry in vivo. Methods in molecular biology (Clifton, N.J.). 438:335-43. Pubmed: 18369768 DOI:10.1007/978-1-59745-133-8_25

Abstract

Bromodeoxyuridine, variously abbreviated as BrdU, BudR, and BrdUrd, is a halogenated thymidine analog that is permanently integrated into the DNA of dividing cells during DNA synthesis in S phase. BrdU can be immunocytochemically detected in vitro and in vivo, allowing the identification of cells that were dividing the period of BrdU exposure. In vivo, it has been used to identify the "birthdate" of cells during development, to examine the fate of postnatally generated cells, and to label cells before transplantation, for subsequent identification.

Related Faculty

Jeffrey Macklis, M.D., D.Sc.Tech.

Macklis Lab

Jeffrey Macklis investigates molecular controls and mechanisms over neuron subtype specification, development, diversity, axon guidance-circuit formation, and pathology in the cerebral cortex. His lab seeks to apply developmental controls toward brain and spinal cord regeneration and directed differentiation for in vitro mechanistic modeling using human assembloids.