Le TP, Vuong LT, Kim AR, Hsu YC, Choi KW. 2016. 14-3-3 proteins regulate Tctp-Rheb interaction for organ growth in Drosophila. Nature communications. 7:11501. Pubmed: 27151460 DOI:10.1038/ncomms11501


14-3-3 family proteins regulate multiple signalling pathways. Understanding biological functions of 14-3-3 proteins has been limited by the functional redundancy of conserved isotypes. Here we provide evidence that 14-3-3 proteins regulate two interacting components of Tor signalling in Drosophila, translationally controlled tumour protein (Tctp) and Rheb GTPase. Single knockdown of 14-3-3ɛ or 14-3-3ζ isoform does not show obvious defects in organ development but causes synergistic genetic interaction with Tctp and Rheb to impair tissue growth. 14-3-3 proteins physically interact with Tctp and Rheb. Knockdown of both 14-3-3 isoforms abolishes the binding between Tctp and Rheb, disrupting organ development. Depletion of 14-3-3s also reduces the level of phosphorylated S6 kinase, phosphorylated Thor/4E-BP and cyclin E (CycE). Growth defects from knockdown of 14-3-3 and Tctp are suppressed by CycE overexpression. This study suggests a novel mechanism of Tor regulation mediated by 14-3-3 interaction with Tctp and Rheb.

Related Faculty

Photo of Ya-Chieh Hsu

Ya-Chieh Hsu studies the principles and molecular nature of cell-cell interactions governing development, regeneration, and injury repair in the skin.

Search Menu