Galvin A, Weglarz M, Folz-Donahue K, Handley M, Baum M, Mazzola M, Litwa H, Scadden DT, Silberstein L. Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry. Curr Protoc Cytom. 2019 01; 87(1):e50.

Citation

Galvin A, Weglarz M, Folz-Donahue K, Handley M, Baum M, Mazzola M, Litwa H, Scadden DT, Silberstein L. 2019. Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry. Current protocols in cytometry. 87(1):e50. Pubmed: 30335223 DOI:10.1002/cpcy.50

Abstract

Maintenance of hematopoietic stem cell (HSC) quiescence is critical for self-renewal and differentiation into mature lineages. Therefore, the ability to reliably detect abnormal HSC cycling is essential for experiments that seek to investigate abnormalities of HSC function. The ability to reproducibly evaluate cell cycle status in a rare cell subset requires careful optimization of multiple parameters during cell preparation and sample processing. Here, we describe a method where data acquisition parameters and fluorochrome combination for long-term HSC staining have been specifically designed for concurrent use with DAPI and Ki-67 antibodies. © 2018 by John Wiley & Sons, Inc.

Related Faculty

David Scadden, M.D.

Scadden Lab

David Scadden’s laboratory is dedicated to discovering the principles governing blood cell production, with the ultimate goal of guiding the development of therapies for blood disorders and cancer.