Zhong TP, Kaphingst K, Akella U, Haldi M, Lander ES, Fishman MC. Zebrafish genomic library in yeast artificial chromosomes. Genomics. 1998 Feb 15; 48(1):136-8.

Citation

Zhong TP, Kaphingst K, Akella U, Haldi M, Lander ES, Fishman MC. 1998. Zebrafish genomic library in yeast artificial chromosomes. Genomics. 48(1):136-8. Pubmed: 9503028

Abstract

We have constructed a zebrafish yeast artificial chromosome (YAC) library using genomic DNA isolated from the inbred AB zebrafish strain. The average insert size is 470 kb, estimated from analysis of 155 random selected YACs. The library consists of 17,000 clones, providing about a 4.7-fold coverage of zebrafish genome. The YAC clones have been arrayed in individual wells of 96-well microplates and also pooled to permit rapid polymerase chain reaction screening of the entire library. We have also found that the YAC ends can be easily rescued and sequenced from pRML1/pRML2-based mini-YAC clones.

Related Faculty

Mark C. Fishman, M.D.

Fishman Lab

Mark C. Fishman’s group studies the heart-brain connection. They employ a range of genetic, developmental, and neurobiological tools in zebrafish to understand what the heart tells the brain, and how critical internal sensory systems adjust homeostatic and somatic behaviors, including social interactions.