Silva-García CG, Lanjuin A, Heintz C, Dutta S, Clark NM, Mair WB. Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans. G3 (Bethesda). 2019 May 07.

Citation

Silva-García CG, Lanjuin A, Heintz C, Dutta S, Clark NM, Mair WB. 2019. Single-Copy Knock-In Loci for Defined Gene Expression in. G3 (Bethesda, Md.). Pubmed: 31064766

Abstract

We have generated single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical and effective approach for generating single-copy ectopic transgenes in .

Related Faculty

William Mair, Ph.D.

Will Mair investigates the molecular pathways underpinning the aging process, with the goal of using this knowledge to develop novel therapeutic strategies to treat age-onset disorders.