Herring A, Messana A, Bara AM, Hazelbaker DZ, Eggan K, Barrett LE. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem Cell Res. 2016 09; 17(2):430-432.

Citation

Herring A, Messana A, Bara AM, Hazelbaker DZ, Eggan K, Barrett LE. 2016. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem cell research. 17(2):430-432. Pubmed: 27879218 DOI:10.1016/j.scr.2016.09.009

Abstract

Here, we generated a biallelic mutation in the TLE1 (Transducin Like Enhancer of Split 1) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The homozygous knockout cell line, TLE1-464-G04, displays loss of TLE1 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.

Related Faculty

Kevin Eggan, Ph.D.

Eggan Lab

Kevin Eggan investigates the mechanisms that cause motor neuron degeneration in Amyotrophic Lateral Sclerosis (ALS), and seeks to translate new discoveries into new therapeutic options for patients.