Citation

Chetty S, Engquist EN, Mehanna E, Lui KO, Tsankov AM, Melton DA. 2015. A Src inhibitor regulates the cell cycle of human pluripotent stem cells and improves directed differentiation. The Journal of cell biology. 210(7):1257-68. Pubmed: 26416968 DOI:10.1083/jcb.201502035

Abstract

Driving human pluripotent stem cells (hPSCs) into specific lineages is an inefficient and challenging process. We show that a potent Src inhibitor, PP1, regulates expression of genes involved in the G1 to S phase transition of the cell cycle, activates proteins in the retinoblastoma family, and subsequently increases the differentiation propensities of hPSCs into all three germ layers. We further demonstrate that genetic suppression of Src regulates the activity of the retinoblastoma protein and enhances the differentiation potential of hPSCs across all germ layers. These positive effects extend beyond the initial germ layer specification and enable efficient differentiation at subsequent stages of differentiation.
© 2015 Chetty et al.

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Photo of Doug Melton

Doug Melton is pursuing a cure for type 1 diabetes. His lab studies the developmental biology of the pancreas, using that information to grow and develop pancreatic cells (islets of Langerhans). In parallel, they investigate ways to protect beta cells from autoimmune attack.

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