Bara AM, Messana A, Herring A, Hazelbaker DZ, Eggan K, Barrett LE. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem Cell Res. 2016 09; 17(2):441-443.

Citation

Bara AM, Messana A, Herring A, Hazelbaker DZ, Eggan K, Barrett LE. 2016. Generation of a TLE3 heterozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem cell research. 17(2):441-443. Pubmed: 27879221 DOI:10.1016/j.scr.2016.09.008

Abstract

Here, we generated a monoallelic mutation in the TLE3 (Transducin Like Enhancer of Split 3) gene using CRISPR-Cas9 editing in the human embryonic stem cell (hESC) line WA01. The heterozygous knockout cell line, TLE3-447-D08-A01, displays partial loss of TLE3 protein expression while maintaining pluripotency, differentiation potential and genomic integrity.

Related Faculty

Kevin Eggan, Ph.D.

Eggan Lab

Kevin Eggan investigates the mechanisms that cause motor neuron degeneration in Amyotrophic Lateral Sclerosis (ALS), and seeks to translate new discoveries into new therapeutic options for patients.