A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls. Methods Mol Biol. 2023; 2562:335-349.

Citation

Sousounis K, Courtemanche K, Whited JL. 2023. A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls. Methods in molecular biology (Clifton, N.J.). 2562:335-349. Pubmed: 36272086 DOI:10.1007/978-1-0716-2659-7_22

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function.

Related Faculty

Jessica Whited, Ph.D.

Whited Lab

Jessica Whited studies limb regeneration in axolotl salamanders. Her lab develops tools to manipulate gene expression during limb regeneration, and explores signaling events following wound healing that initiate the regenerative process.