Our laboratory is directed toward both 1) understanding the molecular controls over neuron sub-type specification and development in the cerebral cortex, and 2) applying developmental controls toward brain and spinal cord repair—specifically, the cellular repair of complex cerebral cortex and cortical output circuitry (in particular, cortico-spinal motor neuron (CSMN) circuitry that degenerates in ALS and other “upper motor neuron” degenerative diseases, and whose injury is centrally involved in loss of motor function in spinal cord injury).
We focus on neocortical projection neuron development and sub-type specification; neural progenitor / “stem cell” biology; induction of adult neurogenesis (the birth of new neurons from within); and directed neuronal differentiation and development of connectivity via molecular manipulation of neural progenitors within murine neocortex. The same biology informs understanding of neuronal subtype specificity of involvement in human neurodegenerative and developmental diseases, in particular ALS / motor neuron disease, PLS, HSPs, Huntington's disease, autism spectrum disorders, and Rett Syndrome.
Welcome to the Macklis Lab at Harvard University in Cambridge, Massachusetts!
As you approach our lab walking through the Harvard Yard, you will find the Bauer Laboratory Building.
On the 1st floor you can find the Macklis lab! Even before walking in, you'll find us at our lab benches through the glass windows. Looking around the lab, you'll see us working on a diversity of projects.
Some of these projects involve molecular biology, biochemistry, and immunolabeling...
We analyze cells, tissue sections, and even whole-mount brains in our tissue culture and histology rooms.
Plus, plenty of time is spent in the darkness of our microscopy rooms, acquiring snapshots and montages of our precious samples..
Finally, much of the magic happens in the room shown below. Here we perform various types of brain surgery using asceptic technique: ultrasound guided and stereotaxic labeling, electroporation, and other in vivo manipulations. We also share an electrophysiology rig with the Murthy lab, to study the functional integration of neocortical neurons.
When we're not at our benches, you can find us.... working at our desks, brainstorming in the small conference room, or kicking back in our lounge.
We hope to see you soon!